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为了筛选适宜的脲酶抑制剂与硝化抑制剂组合,本试验通过在室内模拟培养条件下,研究不同浓度的NBPT与Nitrapyrin配比对石灰性土壤氮素转化的影响。结果表明:尿素施入土壤1 d后80%已迅速水解,在5 d后已完全水解。在1-14 d不同浓度的NBPT均可显著抑制尿素水解(P<0.05),且10%o NBPT可高效作用14 d,其脲酶抑制率为51%。添加抑制剂延缓了铵态氮的转化过程,各施肥处理土壤NH_4~+-N含量分别在第7、14、21天达到峰值,而各抑制剂处理NO_3~--N含量呈线性缓慢上升趋势。添加抑制剂各处理均降低了土壤氮素损失,相比单施尿素处理分别降低14%、22%、26%、28%(P<0.05)。NBPT 5‰+Nitrapyrin可有效抑制尿素水解,减缓氮素转化过程,减少石灰性土壤氮素损失。
In order to screen suitable combination of urease inhibitor and nitrification inhibitor, this experiment studied the effects of different concentrations of NBPT and Nitrapyrin on the transformation of nitrogen in calcareous soil under laboratory simulated culture conditions. The results showed that 80% of urea was rapidly hydrolyzed after being applied to soil for 1 d, and completely hydrolyzed after 5 d. NBPT at different concentrations from 1 to 14 days significantly inhibited urea hydrolysis (P <0.05), and 10% o NBPT was effective for 14 days with a urease inhibition of 51%. The addition of inhibitors slowed the conversion of ammonium nitrogen, and the NH_4 ~ + -N content of the fertilizers reached the peak on the 7th, 14th and 21st day, respectively, while the NO_3 ~ --N content of each inhibitor showed a linear and slowly increasing trend . Compared with the urea treatment, the nitrogen losses in soil increased by 14%, 22%, 26% and 28%, respectively (P <0.05). NBPT 5 ‰ + Nitrapyrin can effectively inhibit the hydrolysis of urea, slow the process of nitrogen transformation and reduce the nitrogen loss of calcareous soil.