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目的 了解粒细胞集落刺激因子(G- CSF)对顺铂对卵巢癌细胞的细胞毒作用的影响;方法 采用XTT 比色法检测不同浓度顺铂对卵巢癌细胞株SKOV3、3AO 细胞生长影响;以及50 mg/L 顺铂分别与不同浓度G- CSF 共同使用对SKOV3、3AO细胞生长的影响。结果 50 mg/L 顺铂单独使用和与不同浓度G- CSF 共同使用24 h 对SKOV3、3AO 细胞生长的影响无显著性差异。结论 G- CSF 对顺铂对SKOV3、3AO 细胞的细胞毒作用无显著影响。
Objective To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on the cytotoxicity of cisplatin on ovarian cancer cells. Methods XTT assay was used to detect the effect of different concentrations of cisplatin on the proliferation of ovarian cancer cell line SKOV3 and 3AAO cells. Effects of 50 mg / L cisplatin and different concentrations of G-CSF on SKOV3,3AO cell growth. Results There was no significant difference in the effects of 50 mg / L cisplatin alone or in combination with different concentrations of G-CSF for 24 h on SKOV3,3AO cell growth. Conclusion G-CSF has no significant effect on the cytotoxicity of cisplatin on SKOV3,3AO cells.