论文部分内容阅读
目的探讨慢性乙型肝炎(CHB)患者血清中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)和白细胞介素-10(IL-10)与HBV-DNA载量关系,为CHB治疗提供理论依据。方法采用酶联免疫吸附实验(ELISA)测定血清中IFN-γ、IL-4和IL-10的水平,荧光定量PCR法测血清HBV-DNA载量。结果 IFN-γ水平与HBV-DNA载量呈负相关(r=-0.290,P<0.01);IL-4和IL-10水平与HBV-DNA载量正相关(r=0.320,r=0.352,P<0.05)。结论血清中细胞因子IFN-γ、IL-4和IL-10与HBV-DNA复制密切相关。
Objective To investigate the relationship between serum levels of IFN-γ, interleukin-4 (IL-4) and interleukin-10 (IL-10) and HBV DNA in patients with chronic hepatitis B , Provide a theoretical basis for the treatment of CHB. Methods Serum levels of IFN-γ, IL-4 and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). The serum HBV DNA level was detected by fluorescence quantitative PCR. Results The level of IFN-γ was negatively correlated with HBV DNA load (r = -0.290, P <0.01). The levels of IL-4 and IL-10 were positively correlated with HBV- P <0.05). Conclusion Serum cytokines IFN-γ, IL-4 and IL-10 are closely related to HBV-DNA replication.