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目的:研究蚂蟥酶解物不同部位的抗凝活性。方法:分别采用有机溶剂沉淀法、超滤法以及C18反相色谱法将蚂蟥酶解物分为不同有机溶剂沉淀部位、大分子和小分子部位、强极性和弱极性部位,以凝血酶时间为抗凝活性指标测定各部位的抗凝活性;同时采用外翻肠囊模型,研究不同部位经大鼠肠吸收后的抗凝活性差异。结果:利用有机溶剂沉淀法所得各部位的中、高剂量组均有抗凝活性(P<0.05);分级超滤所得分子量>10KD的部位具有显著抗凝活性(P<0.01),却不易被肠吸收,分子量<10KD的部位虽然抗凝活性较低(P<0.05),但较易通过肠黏膜;强极性部位和弱极性部位均有抗凝活性(P<0.05,P<0.01),但强极性部位活性更显著,更易透过肠黏膜。结论:蚂蟥酶解物活性分散,成分复杂,其发挥抗凝作用的成分可能为一些小分子大极性多肽类成分。
Objective: To study the anticoagulant activity of different parts of leech lysate. Methods: The organic solvent precipitation, ultrafiltration and C18 reverse phase chromatography were used to separate the leech hydrolyzate into different organic solvent precipitates, macromolecules and small molecules, strongly polar and weak polar sites, thrombin Time anticoagulant activity determination of anticoagulant activity of various parts of the site; at the same time using the valgus gut model to study different parts of the rat intestinal absorption after anticoagulant activity differences. Results: The anticoagulant activity of medium and high dose groups (P <0.05) was obtained by using organic solvent precipitation method. The fractions with molecular weight> 10KD obtained by fractional ultrafiltration had significant anticoagulant activity (P <0.01), but not easily (P <0.05, P <0.01), although the anticoagulant activity was lower (P <0.05) in intestinal absorption and molecular weight <10KD, but more likely to pass through intestinal mucosa. , But the more active parts of the more polar, more easily through the intestinal mucosa. Conclusion: The active components of leech hydrolyzate are scattered and the components are complex. The anticoagulant components of leech may be some components of large polar peptides.