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本研究旨在探讨羟基磷灰石/磷酸三钙(HA/TCP)生物陶瓷材料植入小鼠体内骨诱导过程中新生血管的形成过程。将HA/TCP材料植入小鼠肌肉中,手术后2、4、6、8、10和12周,收获材料制备连续切片,通过观察HE染色图片动态描述新生血管形成过程,并定量分析新生血管形成百分比。结果显示新生血管形成是一个连续、动态的过程,其在第2周时就大量生成,4周时呈增长趋势,6周时达到峰值,8周后逐步减少。本研究结果为提高骨组织工程成功率提供了思路,也从血管化方面揭示了骨诱导形成的机制。
The purpose of this study was to investigate the formation of neovascularization during osteoinduction in mice implanted with hydroxyapatite / tricalcium phosphate (HA / TCP) bioceramic material. The HA / TCP material was implanted into the muscle of mice. After harvesting for 2, 4, 6, 8, 10 and 12 weeks, serial sections were harvested and the neovascularization was dynamically described through the observation of HE staining. The neovascularization Form a percentage. The results showed that neovascularization was a continuous and dynamic process. The neovascularization was extensively produced in the second week, increased in the fourth week, peaked in the sixth week and gradually decreased in the eighth week. The results of this study provide ideas for improving the success rate of bone tissue engineering, and also reveal the mechanism of osteoinduction from the vascularization aspect.