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目的探讨人宫颈癌基因(human cervical cancer oncogene,HCCR)在人胃癌组织和胃癌细胞株中的表达及其与临床病理特征的关系,初步评价HCCR在胃癌发生中的作用及其意义,为寻找胃癌新的诊断标志物另辟新径。方法采用Western blot方法检测SGC-7901、BGC-823胃癌细胞系中HCCR蛋白表达;采用免疫组化法检测30例胃癌患者的癌组织和10例癌旁正常组织中HCCR蛋白表达。结果HCCR蛋白在两种不同分化程度的胃癌细胞株中均有阳性表达;胃癌组织中HCCR阳性表达率为73.3%(22/30),其表达位于细胞的胞膜和胞质,10例癌旁正常组织均为阴性表达,两者差异具有统计学意义(P<0.01)。HCCR的表达与胃癌患者的年龄、性别、浸润深度、组织分型、肿瘤分化程度及有无淋巴结转移均无显著相关性(P>0.05)。结论HCCR与胃癌的发生密切相关,可能是反映早期胃癌发生的重要生物学指标之一。
Objective To investigate the expression of human cervical cancer oncogene (HCCR) in human gastric cancer and gastric cancer cell lines and its relationship with clinicopathological features. To evaluate the role and significance of HCCR in gastric carcinogenesis, New diagnostic markers open a new path. Methods Western blot was used to detect the expression of HCCR protein in SGC-7901 and BGC-823 gastric cancer cell lines. HCCR protein expression was detected by immunohistochemistry in 30 cases of gastric cancer and 10 cases of adjacent normal tissues. Results The expression of HCCR protein in both gastric cancer cell lines with different differentiation degrees was positive. The positive expression rate of HCCR in gastric cancer tissues was 73.3% (22/30). The expression of HCCR protein was located in the cell membrane and cytoplasm, The normal tissues were negative, the difference between the two was statistically significant (P <0.01). There was no significant correlation between the expression of HCCR and the age, sex, depth of invasion, histological type, tumor differentiation and lymph node metastasis in gastric cancer patients (P> 0.05). Conclusions HCCR is closely related to the occurrence of gastric cancer and may be one of the important biological indicators of early gastric cancer.