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目的分析不同病变胃粘膜细胞内端粒长度的差异,以及细胞内 DNA 的含量,并探讨端粒行为异常、细胞内 DNA 含量与胃粘膜癌变的关系.方法对172例内镜活检和45例胃癌手术标本,应用Southern 杂交分析细胞内端粒长度,应用流式细胞术测定细胞内 DNA 含量.结果在172例内镜活检标本中,正常胃粘膜,慢性浅表性胃炎,伴0.1,2度肠化的慢性萎缩性胃炎和胃癌组织的端粒长度分别是10.4Kb±0.2Kb,9.9Kb±0.4Kb.9.8Kb±1.2Kb,8.6Kb±1.0Kb,6.2Kb±1.2Kb,5.9Kb±2.6Kb.在45例胃癌手术标本中结果相似.流式细胞术分析细胞内 DNA 含量的结果是,在门诊内镜活检标本中,正常胃粘膜,慢性浅表性胃炎,伴0,1,2度肠化的慢性萎缩性胃炎和胃癌组织的异倍体 DNA检出率分别为0.00%,0.00%,0.00%,10.00%,12.50%,33.33%在45例胃癌手术切除标本中结果也相似.而且异倍体细胞内的端粒长度明显短于二倍体细胞内的端粒长度,同时异倍体细胞中的端粒长度与 DNA 指数呈负相关(r=0.91,P<0.01),也就是端粒越短 DNA 指数越高.结论端粒长度从正常胃粘膜、不同程度肠化胃粘膜到癌变胃粘膜而逐渐缩短.在正常胃粘膜和慢性浅表性胃炎中未检出异倍体 DNA,从1度肠化、2度肠化到癌变的胃粘膜异倍体DNA 检出率逐渐增高,而且在异倍体细胞中端粒长度和 DNA指数呈负相关,推测可能存在端粒愈短 DNA 扩增愈活跃的情况,端粒缩短伴有 DNA 指数增加可能是胃癌发生的预兆.
OBJECTIVE: To analyze the difference of telomere length and DNA content in gastric mucosal cells of different pathological types and to explore the relationship between abnormal telomere activity and DNA content in gastric mucosa.Methods 172 cases of endoscopic biopsy and 45 cases of gastric cancer Surgical specimens were analyzed by Southern blotting, and the intracellular telomere length was analyzed by flow cytometry.Results In 172 cases of endoscopic biopsy specimens, normal gastric mucosa, chronic superficial gastritis, The telomere length of the transformed chronic atrophic gastritis and gastric cancer tissues were 10.4Kb ± 0.2Kb, 9.9Kb ± 0.4Kb.9.8Kb ± 1.2Kb, 8.6Kb ± 1.0Kb, 6.2Kb ± 1.2Kb, 5.9Kb ± 2.6Kb, respectively .The results were similar in 45 cases of gastric cancer surgery.Flow cytometry analysis of intracellular DNA content results in endoscopic biopsy specimens, the normal gastric mucosa, chronic superficial gastritis, with 0,1,2 degree intestine The detection rates of aneuploidy in patients with chronic atrophic gastritis and gastric cancer were 0.00%, 0.00%, 0.00%, 10.00%, 12.50% and 33.33%, respectively, and the results were similar in 45 cases of gastric cancer resected specimens The telomere length in ploidy cells is significantly shorter than the telomere length in diploid cells, The telomere length was negatively correlated with DNA index (r = 0.91, P <0.01), that is, the shorter the telomere, the higher the DNA index.Conclusion The telomere length varies from normal gastric mucosa, varying degrees of intestinal mucosa to carcinogenesis Gastric mucosa and gradually shortened in normal gastric mucosa and chronic superficial gastritis was not detected aneuploid DNA, from 1 degree intestinal metaplasia, 2 degree intestinal metaplasia to cancer gastric mucosal aneuploidy DNA detection rate gradually increased, Furthermore, the telomere length in aneuploid cells was negatively correlated with the DNA index. It is speculated that there may be more active DNA amplification in telomere. Shortening of telomere with DNA index increase may be a sign of gastric cancer.