论文部分内容阅读
目的：了解鱼精蛋白应用于血管内皮生长因子受体介导的靶向性非病毒载体的可行性。方法：利用ＧＶ１，ＧＶ２靶向性非病毒载体来比较多聚赖氨酸与鱼精蛋白对靶向性基因转移复合体携带ＤＮＡ的能力及体外基因转移效率的影响。结果：在Ａ３７５细胞中，鱼精蛋白与多聚赖氨酸参与形成的复合体基因导入率都为５０％左右。在ＡＢＡＥ细胞中，鱼精蛋白参与形成的复合体基因导入率只有２０％左右，而多聚赖氨酸可达７０％左右。鱼精蛋白参与形成的复合体导入引起的基因表达在转染后第６天达到高峰，传代培养不影响基因的表达水平；多聚赖氨酸在转染后第７天达到高峰，传代培养使基因的导入率明显下降。结论：鱼精蛋白在血管内皮生长因子受体介导的基因转移系统中的应用具有一定的限制性。
Objective: To investigate the feasibility of protamine in vascular endothelial growth factor receptor-mediated targeting of non-viral vectors. Methods: GV1 and GV2-targeting non-viral vectors were used to compare the effects of polylysine and protamine on DNA carrying capacity of target gene transfer complexes and gene transfer efficiency in vitro. RESULTS: In A375 cells, the introduction rate of the complex gene formed by protamine and polylysine was about 50%. In ABAE cells, the introduction rate of the complex gene involved in the formation of protamine is only about 20%, and polylysine can reach about 70%. The gene expression induced by proteoglycans involved in the formation of complexes reached a peak on the 6th day after transfection, and subcultured cultures did not affect the expression level of genes; polylysine peaked on the 7th day after transfection, and subculture resulted in The introduction rate of the gene was significantly decreased. Conclusion: The application of protamine in vascular endothelial growth factor receptor-mediated gene transfer system has certain limitations.