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目的:研究与纯钛相比,经羟基磷灰石表面改性处理的纯钛是否能增加兔角膜基质成纤维细胞在其表面的粘附和增殖。方法:利用生物活性的羟基磷灰石对纯钛进行表面改性。将第4代兔角膜基质成纤维细胞接种于羟基磷灰石表面改性的钛、纯钛及玻璃表面,24,48,72h后,使用丫啶橙染色的方法观察材料表面细胞的粘附和增殖情况,电镜观察材料表面的细胞形态。结果:各个时间点,羟基磷灰石表面改性的钛表面的细胞数都多于其他材料(P<0.05)。24h,羟基磷灰石表面改性的钛表面和玻璃表面的细胞扩展面积要大于纯钛表面。72h电镜观察发现羟基磷灰石表面改性的钛表面的细胞扩展面积最大,细胞张力丝最长。结论:与纯钛相比,经羟基磷灰石表面改性处理的纯钛可增加兔角膜基质成纤维细胞在其表面粘附和增殖
OBJECTIVE: To investigate whether pure titanium modified by hydroxyapatite can increase the adhesion and proliferation of rabbit corneal fibroblasts on its surface compared with pure titanium. Methods: The biologically active hydroxyapatite was used to modify the surface of pure titanium. The 4th generation rabbit corneal stroma fibroblasts were inoculated on the surfaces of hydroxyapatite modified titanium, pure titanium and glass. After 24, 48 and 72 hours, the acridine orange staining was used to observe the cell surface adhesion and Proliferation, electron microscopy of the cell surface morphology of the material. Results: At each time point, the number of cells on the surface of hydroxyapatite-modified titanium was more than that of other materials (P <0.05). 24h, hydroxyapatite surface modification of the titanium surface and the glass surface of the cell expansion area is larger than pure titanium surface. 72h electron microscopy showed that hydroxyapatite surface modified titanium surface of the largest cell expansion area, the longest cell tension silk. Conclusion: Compared with pure titanium, pure titanium treated with hydroxyapatite can increase the adhesion and proliferation of rabbit corneal stroma fibroblasts