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目的:观察益智饮对β-淀粉样肽(Aβ)1-42致阿尔茨海默病(AD)模型大鼠学习记忆能力的影响及抗氧化作用。方法:Aβ1-42注射建立AD大鼠模型,分别给予不同剂量益智饮治疗。采用Morris水迷宫检测学习记忆能力、免疫组化检测大鼠脑组织Aβ蛋白表达情况;并测定脑组织超氧化物歧化酶(SOD)含量、羟自由基抑制率。结果:与模型组相比,益智饮高、中、低剂量组的逃避潜伏期缩短(P<0.01);以高剂量组最优;高、中剂量组脑组织内Aβ聚集表达减少。益智饮各剂量组及阳性对照组大鼠脑组织SOD活性均高于模型组(P<0.01);高、中剂量组的羟自由基抑制率显著升高(P<0.01)。结论:益智饮能较好改善AD大鼠的学习记忆能力,减少脑内Aβ的聚集。其机制可能与通过升高SOD的活性和抑制羟自由基的生成有关。
OBJECTIVE: To observe the effect of Yizhiyin on learning and memory abilities of Alzheimer’s disease (AD) rats induced by β-amyloid peptide (Aβ) 1-42 and its antioxidation. METHODS: AD rat model was established by Aβ1-42 injection and treated with different doses of Zhiyin respectively. Morris water maze was used to test the learning and memory abilities. Immunohistochemistry was used to detect the expression of Aβ protein in brain tissue of rats. The contents of superoxide dismutase (SOD) and hydroxyl radical inhibitory rate in brain tissue were measured. RESULTS: Compared with the model group, the escape latency of the high-, medium-, and low-dose groups of Zhiyin Decoction was shortened (P<0.01). The high-dose group was the best and the Aβ aggregation in the brain tissue of the high- and middle-dose groups was decreased. The SOD activity in the brain of each dose group and positive control group was higher than that of the model group (P<0.01), and the hydroxyl radical inhibitory rate was significantly higher in the high and middle dose groups (P<0.01). Conclusion: Yizhi Yin can better improve the learning and memory ability of AD rats and reduce the accumulation of Aβ in brain. Its mechanism may be related to increasing SOD activity and inhibiting the production of hydroxyl radicals.