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目的:探讨信号转导和转录激活因子3(STAT3)及磷酸化STAT3(P-STAT3)在喉癌组织中的表达情况及活化STAT3蛋白表达与喉癌临床病理特征的关系。方法:用免疫组织化学法检测50例手术切除的喉癌组织(喉癌组)和10例癌旁正常组织(对照组)中STAT3及P-STAT3蛋白的表达。结果:喉癌组中有36例(72.0%)STAT3蛋白表达阳性,29例(58.0%)P-STAT3蛋白表达阳性,对照组均为阴性表达(均P<0.01)。STAT3主要表达在细胞质,P-STAT3主要表达在细胞核内;STAT3及P-STAT3在低分化、临床分期较晚和有淋巴结转移的喉癌患者中阳性表达率高(P<0.05或P<0.01)。结论:喉癌组织中存在STAT3和P-STAT3的高表达,并与临床病理分期和淋巴结转移有关;STAT3的表达可作为判定喉癌生物学行为的一个指标。
Objective: To investigate the expression of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (P-STAT3) in laryngeal carcinoma and the relationship between the expression of activated STAT3 protein and clinicopathological features of laryngeal carcinoma. Methods: The expressions of STAT3 and P-STAT3 protein in 50 cases of laryngeal carcinoma (LSCC) and 10 cases of adjacent normal tissues (control group) were detected by immunohistochemistry. RESULTS: Thirty-six patients (72.0%) had positive STAT3 protein expression in the laryngeal squamous cell carcinoma group, and 29 (58.0%) had positive P-STAT3 protein expression, all of which were negative in the control group (all P <0.01). STAT3 was mainly expressed in the cytoplasm and P-STAT3 was mainly expressed in the nucleus. STAT3 and P-STAT3 were positively expressed in laryngeal carcinoma with poorly differentiated, late clinical stage and lymph node metastasis (P <0.05 or P <0.01) . Conclusion: There is a high expression of STAT3 and P-STAT3 in laryngeal carcinoma, which is related to clinicopathologic stage and lymph node metastasis. The expression of STAT3 may be used as an index to judge the biological behavior of laryngeal cancer.