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鉴别用选择性培养基DYS配方是,取细菌学蛋白胨15克、水解干酪素5克、牛胆粉8.5克、去氧胆酸钠20克、氯化钠5克、阿拉伯糖10克、盐酸精氨酸6.5克、盐酸赖氨酸6.5克、中性红0.04克、琼脂20克及蒸溜水1000毫升。将成份混合,浸泡5分钟,加热并不断摇动,使其溶解。冷至45℃时,校正pH至7.1,倾碟,4℃保存备用。因培养基含有胆盐可提高选择性。加入阿拉伯糖、精氨酸和赖氨酸以区别变形菌、假单胞菌和其它肠杆菌。分离时,将大便标本直接涂布平板,23℃培养36小时,检查有无结肠炎耶尔森氏菌菌落。根据菌落颜色,将结肠
Identification with selective medium DYS formula is to take bacterial peptone 15 grams, 5 grams of casein hydrolysis, oxen gall powder 8.5 grams, sodium deoxycholate 20 grams, 5 grams of sodium chloride, 10 grams of arabinose, hydrochloric acid fine 6.5 g of lysine, 6.5 g of lysine hydrochloride, 0.04 g of neutral red, 20 g of agar and 1000 ml of distilled water. The ingredients are mixed, soaked for 5 minutes, heated and constantly shaken to dissolve. Cold to 45 ° C, the pH was adjusted to 7.1, tilted dish, 4 ° C save spare. Because the medium contains bile salts to improve selectivity. Adsorption of arabinose, arginine and lysine to distinguish Proteobacteria, Pseudomonas and other Enterobacteriaceae. At the time of separation, the stool specimen was directly coated on the plate and incubated at 23 ° C for 36 hours to examine for the presence or absence of colitis Yersinia colonies. According to colony color, colon