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用30只成年豚鼠的腹腔神经节在恒冷箱内制成连续切片,先按 De La Torre 乙醛酸法诱发荧光,对 SIF 细胞进行观察定位,随即在同一张切片上进行酶及其它组织化学反应。在所使用的反应条件下,与主神经元对比,SIF 细胞具有较强的 Mg~(++)激活的三磷酸腺苷酶,较弱的单胺氧化酶、乳酸脱氢酶及非特异性酯酶活性以及完全呈阴性反应的乙酰胆碱酯酶活性。用同时偶联法显示出SIF 细胞的酸性磷酸酶活性较弱,用铅法在大部分 SIF 细胞得到同样的结果。SIF 细胞质内脂类和核酸的含量均较主神经元少。此外,在荧光显微镜下可见到 SIF 细胞成团分布于血管周围,少数SIF 细胞发出长达100微米的细长突起。SIF 细胞的这些组织化学特性和形态特点表明它们可能以不同于主神经元的方式行使功能。内分泌或旁分泌可能是它们行使功能的主要方式。
The celiac ganglia of 30 adult guinea pigs were made into serial sections in a constant temperature cold box. Fluorescence was first induced by De La Torre glyoxylate method, and SIF cells were observed and located. Then, enzyme and other histochemicals were performed on the same slice reaction. Under the reaction conditions used, compared with the main neurons, SIF cells had stronger Mg ~ (++) -activated ATPase, weaker monoamine oxidase, lactate dehydrogenase and nonspecific esterase activity as well as completely negative Reaction of acetylcholinesterase activity. SIF cells showed weak acid phosphatase activity by the simultaneous coupling method, and the same result was obtained by using lead method in most SIF cells. SIF cytoplasmic lipid and nucleic acid content than the main neurons less. In addition, SIF cells can be found in clusters around the blood vessels under a fluorescence microscope, with a few SIF cells emitting elongated protrusions of up to 100 microns. These histochemical and morphological features of SIF cells suggest that they may function differently from the main neurons. Endocrine or paracrine may be the main way they function.