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目的 探讨康宁克通在活体中对增生性瘢痕Ⅰ、Ⅲ型胶原合成及降解影响的机理。方法 用免疫组织化学及分子生物学技术 ,对 6例增生性瘢痕局部注射康宁克通 3及 7d后 ,Ⅰ、Ⅲ型胶原蛋白及相应前胶原mRNA的原位表达进行了研究。结果 ①注射 7d后 ,Ⅰ型胶原蛋白量降低 (P <0 0 5 ) ,Ⅲ型胶原蛋白量未见明显降低 (P >0 0 5 )。而Ⅰ、Ⅲ型前胶原mRNA在注射后 3d已被明显抑制 (P <0 0 1) ,至注射后 7d ,表达强度进一步下降。②康宁克通局部注射后对Ⅰ型前胶原mRNA的表达抑制可能比对Ⅲ型前胶原强。③Ⅰ、Ⅲ型前胶原mRNA表达在增生性瘢痕中比正常皮肤强。结论 康宁克通对Ⅰ型胶原的抑制比Ⅲ型胶原强。Ⅰ、Ⅲ型胶原mRNA表达在增生性瘢痕比正常皮肤强
Objective To investigate the mechanism of Corning’s effect on the synthesis and degradation of type Ⅰ and type Ⅲ collagen in hypertrophic scars in vivo. Methods Immunohistochemistry and molecular biology techniques were used to study the in situ expression of type Ⅰ and type Ⅲ collagen and procollagen mRNA after 3 and 7 days of topical hypertrophic scar injection. Results ① After 7 days of injection, the amount of type Ⅰ collagen decreased (P <0 05) and the amount of type Ⅲ collagen did not decrease significantly (P 0 05). However, mRNA of type Ⅰ and type Ⅲ procollagen was significantly inhibited 3d on the 3rd day after injection (P <0.01), and decreased to the level of 7 days after injection. ② Kang Ning Ketong after local injection of type Ⅰ procollagen mRNA expression inhibition may be stronger than type Ⅲ procollagen. ③Ⅰ, Ⅲ procollagen mRNA expression in hypertrophic scar stronger than normal skin. Conclusions Corning’s inhibition of type I collagen is stronger than that of type III collagen. Type Ⅰ and type Ⅲ collagen mRNA expression in hypertrophic scar stronger than normal skin