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目的研究S波段高功率微波(HPM)辐射后乳鼠心肌细胞的损伤效应及其机制。方法采用平均功率密度为5~30mW/cm2的S波段HPM模拟源辐射原代培养的乳鼠心肌细胞,应用流式细胞术、原子力显微镜观察辐射后心肌细胞凋亡和坏死率、[Ca2+]i及细胞膜形态。结果10~30mW/cm2的S波段HPM辐射后6h,心肌细胞凋亡和坏死率增加(P<0·05或P<0·01),且随辐射剂量增大,坏死细胞增多;30mW/cm2HPM辐射后即刻[Ca2+]i升高(P<0·01),细胞膜发生穿孔。结论10~30mW/cm2的S波段HPM辐射可造成心肌细胞凋亡和坏死,[Ca2+]i升高以及细胞膜穿孔,是S波段HPM辐射致心肌细胞损伤的重要机制。
Objective To study the injury effect and mechanism of neonatal rat cardiomyocytes irradiated by high power microwave (HPM) S-band radiation. Methods Primary cultured neonatal rat cardiomyocytes were irradiated with an S-band HPM simulation source with an average power density of 5 ~ 30 mW / cm2. The apoptosis and necrosis of cardiomyocytes were observed by atomic force microscopy and flow cytometry. [Ca2 +] i And cell membrane morphology. Results Six hours after 10 ~ 30mW / cm2 S-band HPM irradiation, the apoptosis and necrosis of cardiomyocytes increased (P <0.05 or P <0.01), and the necrotic cells increased with the increase of radiation dose. Immediately after irradiation, [Ca2 +] i increased (P <0.01) and cell membrane perforation occurred. Conclusion S-band HPM radiation at 10-30 mW / cm2 can induce cardiomyocyte apoptosis and necrosis, increase of [Ca2 +] i and cell membrane perforation, which is an important mechanism of myocardial injury caused by S-band HPM radiation.