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目的探讨高效氯氰菊酯β-CP对MCF-7增殖及雌性幼鼠子宫增重作用的剂效关系。方法采用MTT法检测10-12～10-4mol/L剂量范围内的β-CP对MCF-7细胞的增殖影响的量效关系;采用3 d经皮注射染毒,研究0.1～10 mg/kg剂量范围的β-CP对雌性幼鼠子宫发育影响的剂效关系。结果以β-CP处理24和72 h后,10-10～10-9mol/L范围内,随剂量的增加,MCF-7细胞增殖率(prolieration rate,PR)增高,10-9剂量PR最高,10-9～10-4mol/L范围内,PR随剂量增加而降低,0.1 mg/kgβ-CP剂量组的子宫剥离重系数高于对照组,而1.0和10 mg/kgβ-CP剂量组的子宫剥离重系数要低于对照组(P<0.05)。结论β-CP对MCF-7细胞促增殖作用及促雌性幼鼠子宫增长作用呈现类似倒U型的剂效关系。
Objective To investigate the effect of beta-cypermethrin on the proliferation of MCF-7 and uterine weight gain in young female rats. Methods MTT assay was used to detect the dose-response relationship of β-CP in the dose range of 10-12 ~ 10-4mol / L on the proliferation of MCF-7 cells. After 3 days of transdermal injection, the effects of 0.1 ~ 10 mg / kg Effect of dose range of β-CP on uterine development in female immature rats. Results After treated with β-CP for 24 and 72 h, the proliferization rate (PR) of MCF-7 cells increased and the highest dose of PR (10-9) In the range of 10-9 ~ 10-4mol / L, PR decreased with the increase of dose, and the weight coefficient of uterine detachment in 0.1 mg / kgβ-CP dose group was higher than that in control group. However, in uterus of 1.0 and 10 mg / kgβ-CP dose group Peeling heavy coefficient is lower than the control group (P <0.05). CONCLUSION: The effect of β-CP on the proliferation of MCF-7 cells and on the growth of uterus in female mice show a similar inverted U-shaped effect.