论文部分内容阅读
以线型聚醋酸乙烯酯为致孔剂,由悬浮聚合制得了丙烯腈-醋酸乙烯酯-二乙烯基苯大孔树脂(MR-AV树脂),通过MR-AV树脂的化学改性,合成了聚丙烯偕胺肟-聚乙烯醇大孔球状载体(MR-AVa载体),用FT-IR、SEM、压汞仪和元素分析法研究了树脂及载体的结构与组成,结果表明MR-AVa载体含氮量较高,具有一定的亲水性,且平均孔径大于8,000(?),嗜热菌蛋白酶用戊二醛固定于所得载体之上。以酪蛋白为底物,考察了固定化嗜热菌蛋白酶的催化水解活性及其影响因素,与可溶酶比较,固定化嗜热菌蛋白酶的热稳定性和抗抑制能力显著提高,当载体孔径较大时,所得固定化酶显示出较好的催化活性。
Using linear polyvinyl acetate as porogen, acrylonitrile-vinyl acetate-divinylbenzene macroporous resin (MR-AV resin) was prepared by suspension polymerization. Through the chemical modification of MR-AV resin, The structure and composition of the resin and the carrier were investigated by FT-IR, SEM, mercury porosimetry and elemental analysis. The results showed that the MR-AVa vector Nitrogen content is high, has a certain hydrophilic, and the average pore size is greater than 8,000 (?), Thermolysin with glutaraldehyde fixed on the resulting carrier. Casein as a substrate, investigated immobilized thermostable protease catalytic hydrolysis activity and its influencing factors, compared with the soluble enzyme, immobilized thermolysin thermal stability and anti-inhibitory ability increased significantly when the carrier pore size Larger, the resulting immobilized enzyme showed better catalytic activity.