探讨癌性胸水中肿瘤浸润淋巴细胞(TIL)的生物学特性,便于更好地应用于临床.用低浓度白细胞介素2(IL-2)诱导培养癌性胸水TIL,按常规法计数TIL细胞增殖量,采用流式细胞仪分析TIL细胞表型及MTT法检测其杀伤细胞活性.经低浓度IL-2(10U/ml)诱导培养TIL21d后,TIL增殖倍数为2130±1140,大于1000倍占72%.CD3无显著性变化(P>0.05),CD4,CD8有显著性差异(P<0.01),而CD4/CD8 To investigate the biological characteristics of tumor-infiltrating lymphocytes (TIL) in cancerous pleural effusions, and to facilitate the better clinical application. To use low-level interleukin-2 (IL-2) to induce cancerous pleural effusion TIL, and to count TIL cells according to conventional methods. Proliferation, flow cytometry analysis of TIL cell phenotype and MTT assay to detect the activity of killer cells. After a low concentration of IL-2 (10U/ml) induced TIL21d culture, the proliferation of TIL was 2130 ± 1140, greater than 1000 times the proportion of 72%. There was no significant change in CD3 (P>0.05), there was a significant difference in CD4 and CD8 (P<0.01), while CD4/CD8