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目的了解我国铜绿假单胞菌的氨基糖苷类耐药基因分布状况。方法计算机检索CBM、CNKI、VIP和WanFang Data数据库,收集报道我国铜绿假单胞菌耐氨基糖苷类基因的研究,检索时限均从建库至2012年12月。由2位评价员按照纳入与排除标准筛选文献、提取资料后,采用SPSS 17.0软件进行统计分析。结果共纳入10个省市1 144株耐氨基糖苷类铜绿假单胞菌。氨基糖苷类修饰酶基因aac(3’)-Ⅰ、aac(3’)-Ⅱ、aac(6’)-Ⅰ、aac(6’)-Ⅱ、ant(2’’)-Ⅰ、ant(3’’)-Ⅰ和aph(3’)-Ⅵ在淮河以北地区的检出率分别为13.3%、40.1%、21.6%、40.3%、38.1%、23.7%和2.9%,而淮河以南地区的检出率分别为3.2%、20.2%、15.9%、37.6%、28.3%、28.5%和9.1%。16S rRNA甲基化酶基因rmtA、rmtB和armA检出率分别为20.4%、19.4%和0.7%,而其余16S rRNA甲基化酶基因均未检出。结论氨基糖苷类修饰酶是我国铜绿假单胞菌耐氨基糖苷类药物的主要机制,而16S rRNA甲基化酶是其次要机制。
Objective To understand the distribution of aminoglycoside resistance genes in Pseudomonas aeruginosa in China. Methods The databases of CBM, CNKI, VIP and WanFang Data were searched by computer. The research on the resistance to aminoglycoside genes of Pseudomonas aeruginosa in China was collected from the database to December 2012. Two reviewers screened the documents according to inclusion and exclusion criteria, extracted data, and analyzed the data using SPSS 17.0 software. Results A total of 1 144 resistant aminoglycoside-resistant Pseudomonas aeruginosa strains were collected from 10 provinces. AAC (3 ’) - Ⅰ, aac (3’) - Ⅱ, aac (6 ’) - Ⅰ, aac (6’) - Ⅱ, ant (2 ") - Ⅰ, ant The detection rates of ’’ -Ⅰ and aph (3 ’) - Ⅵ in the area north of Huaihe River were 13.3%, 40.1%, 21.6%, 40.3%, 38.1%, 23.7% and 2.9% The detection rates were 3.2%, 20.2%, 15.9%, 37.6%, 28.3%, 28.5% and 9.1% respectively. The detection rates of 16S rRNA methylase gene rmtA, rmtB and armA were 20.4%, 19.4% and 0.7% respectively, while the other 16S rRNA methylase genes were not detected. Conclusion Aminoglycoside-modifying enzymes are the main mechanism of aminoglycosides-resistant Pseudomonas aeruginosa in China, and 16S rRNA methylase is the secondary mechanism.