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目的:在失血性休克小鼠模型中使用不同的液体复苏,包括等渗盐水(NS)、高渗盐水(HTS)和羟乙基淀粉(HES),比较在不同时间点髓源性抑制细胞(MDSCs)在外周血、脾脏和骨髓组织中分布和分化的情况。创新点:(1)创建失血性休克小鼠模型;(2)将MDSCs引入失血性休克液体复苏后免疫变化的研究中;(3)对骨髓、脾脏和外周血细胞中的MDSCs分布进行研究,并探讨了在失血性休克不同液体复苏后MDSCs的分化趋势,为临床上形成规范的救治方案提供了科学的实践资料。方法:将BALB/c雄性小鼠随机分成四组,除对照组外,其余三组在建立失血性休克小鼠模型后采用不同的液体复苏:NS组、HTS组和HES组。在模型建立后的2、24和72 h分批次处死小鼠,取外周血、脾脏和骨髓细胞组织,通过三色荧光标记流式细胞术进一步分析MDSC细胞含量,以及其两亚组单核髓源性抑制细胞(M-MDSC)和中性粒髓源性抑制细胞(G-MDSC)的比值。结论:HTS可诱导MDSCs在外周血和脾脏中的早期积累,并影响MDSCs分化和分布;而HES对MDSCs的分布影响较小,但对MDSCs在骨髓中的分化影响较大。
OBJECTIVE: Different fluid resuscitation was used in hemorrhagic shock mouse model, including isotonic saline (NS), hypertonic saline (HTS) and hydroxyethyl starch (HES) MDSCs) in peripheral blood, spleen and bone marrow tissue distribution and differentiation. Innovative points: (1) To create a mouse model of hemorrhagic shock; (2) To introduce MDSCs into the immune changes after hemorrhagic shock resuscitation; (3) to study the distribution of MDSCs in bone marrow, spleen and peripheral blood cells The tendency of MDSCs differentiation after hemorrhagic shock resuscitation was discussed, which provided scientific and practical data for the formation of a standardized treatment plan. Methods: BALB / c male mice were randomly divided into four groups. Except for the control group, the other three groups received different fluid resuscitation after establishing the hemorrhagic shock mouse model: NS group, HTS group and HES group. Mice were sacrificed in batches at 2, 24, and 72 h after establishment of the model. Peripheral blood, spleen and bone marrow cells were harvested for further analysis of MDSC cell content by tri-color fluorescent-labeled flow cytometry, The ratio of myeloid derived suppressor cells (M-MDSCs) and neutrophil-derived inhibitory cells (G-MDSCs). CONCLUSION: HTS can induce the early accumulation of MDSCs in peripheral blood and spleen, and affect the differentiation and distribution of MDSCs. HES has little effect on the distribution of MDSCs, but has a great effect on the differentiation of MDSCs in bone marrow.