643名居住在新西兰卡韦劳市的儿童,年龄为1～14岁,在接种乙型肝炎疫苗前8个月对他们的血清标本进行检测表明,HBsAg、抗-HBs和抗-HBc均为阴性。对这些儿童肌肉接种3针Merck药厂的血源性乙型肝炎疫苗,每针2μg,间隔1个月。接种第1针后12个月,用被动血凝试验(PHA)、酶免疫试验(EIA)和放射免疫法(RIA)检测血清标本中乙型 643 children aged from 1 to 14 living in Kaveo, New Zealand, tested their serum samples 8 months prior to the hepatitis B vaccination and found that both HBsAg, anti-HBs and anti-HBc were negative . The children were immunized with a 3-dose Heck Hepatitis B vaccine at 2 doses, 2 μg per needle, at 1-month intervals. At 12 months after inoculation of the first needle, serum samples of type B were detected by passive hemagglutination test (PHA), enzyme immunoassay (EIA) and radioimmunoassay (RIA)