目的:利用HPLC法同时测定当归苦参丸中苦参醇I、苦参酮、槐属二氢黄酮G的含量。方法:ULTI-MATE XB-C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长290 nm。结果:3种成分均达到基线分离,苦参醇I、苦参酮、槐属二氢黄酮G的质量浓度与峰面积分别在8.93～893μg/mL(r1=0.9995)、1.053～105.3μg/mL(r2=0.9996)、5.15～515μg/mL(r3=0.9995)范围内呈良好的线性关系;平均加样回收率分别为100.2%、99.5%、97.2%;RSD分别为1.9%、3.0%、2.0%。结论:该方法准确、简便、灵敏、为当归苦参丸的质量评价提供了依据。 OBJECTIVE: To determine the contents of tanshinone I, matrine and geniposide G in Angelica kushen Pill simultaneously by HPLC method. METHODS: The mobile phase consisted of ULTI-MATE XB-C18 column (4.6 mm × 250 mm, 5 μm) and acetonitrile-water gradient at a flow rate of 1.0 mL / min. Results: The three constituents all achieved baseline separation. The concentrations and peak areas of triterpene I, tanshinone and sophora flavanone G were 8.93 ~ 893μg / mL (r1 = 0.9995), 1.053 ~ 105.3μg / mL (r2 = 0.9996) and 5.15-515μg / mL (r3 = 0.9995). The average recoveries were 100.2%, 99.5% and 97.2% respectively. The RSDs were 1.9%, 3.0% and 2.0% %. Conclusion: The method is accurate, simple and sensitive, and provides the basis for the quality evaluation of Radix Angelica Sinensis.