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目的:采用反相高效液相色谱法测定抗宫炎片中连翘酯苷B和金石蚕苷的含量。方法:使用Diamonsil C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.5%磷酸(18:82),流速1.0mL.min-1,检测波长332nm。结果:连翘酯苷B、金石蚕苷进样量分别在0.12～4.7μg和0.12～4.8μg范围内,与峰面积呈良好线性关系(r=0.9999);连翘酯苷B及金石蚕苷的平均回收率(n=6)分别为99.7%和100.1%,RSD分别为1.8%和1.9%。结论:本法简便、准确,重复性好,可为抗宫炎片的质量控制提供实验依据。
Objective: To determine the contents of Forsythiaside B and Acridine in Kangongyan tablets by reversed-phase high performance liquid chromatography. Methods: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-0.5% phosphoric acid (18:82) at a flow rate of 1.0 mL · min-1. The detection wavelength was 332 nm. Results: The injection volume of forsythiaside B and auromidin were 0.12 ~ 4.7μg and 0.12 ~ 4.8μg, respectively, and showed a good linear relationship with the peak area (r = 0.9999). Forsythiaside B The average recoveries (n = 6) were 99.7% and 100.1%, respectively, with RSDs of 1.8% and 1.9%, respectively. Conclusion: This method is simple, accurate and reproducible. It can provide experimental evidence for the quality control of GongYuanYan tablet.