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目的 观察低剂量辐射诱导胸腺细胞凋亡及细胞周期进程适应性反应的基本规律。方法 实验用X射线照射昆明系雄性小鼠 ,其诱导剂量 (D1 )及其后攻击剂量 (D2 )分别是 75mGy和1 5Gy。D1 和D2 间隔时间分别是 3、6、12、2 4和 6 0h。通过流式细胞仪检测胸腺细胞凋亡和细胞周期进程的变化。结果 当D1 和D2 间隔 3、6和 12h ,D1 +D2 组胸腺细胞凋亡百分数明显低于D2 组(P <0 0 5 ) ,G0 G1 和G2 +M期细胞百分数也不同程度地低于D2 组 ,而S期细胞百分数却明显高于D2 组 (P <0 0 5或P <0 0 1)。结论 上述结果指出 ,D1 在 75mGy ,吸收剂量率为 12 5mGy min ;D2在 1 5Gy ,吸收剂量率为 0 2 87Gy min ;D1 和D2 间隔 3～ 12h ,可在全身照射条件下诱导小鼠胸腺细胞凋亡和细胞周期进程的适应性反应。
Objective To observe the basic rules of adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose radiation. Methods Experiments X-ray irradiation of Kunming male mice, the induced dose (D1) and the subsequent dose of attack (D2) were 75mGy and 15Gy. The interval between D1 and D2 is 3, 6, 12, 24, and 60h, respectively. The changes of thymocyte apoptosis and cell cycle progression were detected by flow cytometry. Results The percentages of apoptotic thymocytes in D1 + D2 group were significantly lower than those in D2 group (P <0.05) at D1, D2 and D2 at 3, 6 and 12h intervals, and the percentage of cells in G0 G1 and G2 + M phase was also lower than D2 Group, while the percentage of cells in S phase was significantly higher than that in D2 group (P <0.05 or P <0.01). Conclusions The above results indicate that D1 is 75 mGy with an absorbed dose rate of 125 mGy min; D2 is 15 Gy with an absorbed dose rate of 0 87 RY min; D1 and D2 are separated by an interval of 3 to 12 h and can induce mouse thymocytes Adaptation of apoptosis and cell cycle progression.