目的建立食品、多维片和饮料中水溶性维生素C、B1,B2,B6,B12,烟酸、烟酰胺、叶酸和脂溶性维生素A、D3、E、K1的反相高效液相色谱同时测定方法。方法样品中水溶性维生素经0.01mol/LHCl超声提取,脂溶性维生素经皂化法提取。饮品直接过滤后测定。色谱条件:色谱柱为C18柱,流动相为0.05mol/LKH2PO4溶液(pH6.0)-甲醇,梯度洗脱流速1.1~1.5ml/min;柱温40℃。结果 12种维生素标准曲线的线性范围:VC为0～5μg,烟酸为0～0.5μg,其余均为0～1μg;相关系数均大于0.996;相对标准偏差均小于5.0%;方法检出限为0.073～0.193μg/ml;加标回收率为75.5%～118.0%。结论所建立的方法快速、简便、灵敏、准确,应用于复合维生素片、米粉、饮料中12种维生素的同时分析,取得了较好结果。 Objective To establish a method for simultaneous determination of water-soluble vitamins C, B1, B2, B6, B12, niacin, nicotinamide, folic acid and fat-soluble vitamins A, D3, E, K1 in food, . Methods The samples of water-soluble vitamins by 0.01mol / LHCl ultrasonic extraction, fat-soluble vitamins by saponification method. Drinks are filtered directly after the determination. Chromatographic conditions: C18 column with a mobile phase of 0.05mol / L KH2PO4 solution (pH6.0) -methanol, gradient elution flow rate of 1.1 ~ 1.5ml / min; column temperature 40 ℃. Results The linear range of 12 standard curves of vitamin was 0 ~ 5μg for VC and 0 ~ 0.5μg for niacin, the rest were 0 ~ 1μg; the correlation coefficients were all greater than 0.996; the relative standard deviations were less than 5.0%; the detection limits were 0.073 ~ 0.193μg / ml; spiked recoveries ranged from 75.5% to 118.0%. Conclusion The established method is rapid, simple, sensitive and accurate. It can be applied to the simultaneous analysis of 12 vitamins in multivitamin tablets, rice noodles and beverages. Good results have been obtained.