论文部分内容阅读
目的 观察外源性金属硫蛋白 (MT)是否具有抗体内自由基损伤及抗衰老效应的能力。 方法 采用小白鼠皮下注射D 半乳糖造成的亚急性衰老模型 ,以阿尼西坦作为阳性对照观察皮下注射不同剂量MT对小鼠在T 型游泳迷宫作业能力的影响及小鼠心、肝、脑组织中丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活性。 结果 MT可明显改善小鼠的学习记忆能力。MT在 1 4mg·Kg-1·d-1剂量时已表现出比阳性对照药物阿尼西坦 (30mg·kg-1·d-1)时更明显的治疗效应 (正确数获得率 ) ;同时MT在 7 0mg·kg-1·d-1时可明显降低MDA含量及提高SOD活性。 结论 MT作为内源性活性氧清除剂对D 半乳糖的致衰老效应有很好的对抗作用。
Objective To observe whether exogenous metallothionein (MT) has the ability of anti-body free radical damage and anti-aging effect. Methods Subacute aging model induced by subcutaneous injection of D-galactose in mice was used. Aniracetam was used as a positive control to observe the effect of subcutaneous injection of different doses of MT on the working ability of mice in the T-swimming maze and the changes of heart, liver and brain Tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results MT can significantly improve learning and memory in mice. MT at the dose of 14 mg · kg-1 · d-1 has shown a more obvious therapeutic effect (the correct number of acquisition) than the positive control drug aniracetam (30 mg · kg-1 · d-1); at the same time, MT at 70 mg · kg-1 · d-1 significantly reduced MDA content and increased SOD activity. Conclusion MT as a reactive oxygen species scavenger has a good antagonistic effect on the aging effect of D-galactose.