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目的 了解EB病毒、bc1 2、系统性红斑狼疮 (SLE)三者之间的关系 ,探讨EB病毒在SLE发生发展中的作用机制。方法 用流式细胞仪双色解析法检测SLE患者外周血B淋巴细胞EB病毒潜伏膜蛋白 (LMP1)和抗凋亡基因bc1 2的表达。结果 活动期患者B淋巴细胞LMP1和bc1 2的表达率均高于稳定期患者 ,两组患者均高于对照组 (P均 <0 0 1) ;SLE患者LMP1+ 细胞bc1 2表达率高于LMP1 细胞 (P <0 0 1) ,且SLE患者外周血B淋巴细胞bc1 2的表达与LMP1的表达呈正相关 (r=0 32 8,P <0 0 5 ) ,与病情积分成正相关 (r=0 339,P <0 0 1)。结论 EB病毒感染B淋巴细胞后 ,通过上调受染细胞bc1 2的表达 ,抑制B淋巴细胞的凋亡 ,参与SLE的发生和发展
Objective To understand the relationship between Epstein-Barr virus (EBV), bc1 2 and systemic lupus erythematosus (SLE) and explore the mechanism of EB virus in the development of SLE. Methods The expression of LMP1 and anti-apoptotic gene bc1 2 in peripheral blood B lymphocytes from patients with SLE were detected by flow cytometry. Results The positive rates of LMP1 and bc1 2 in active lymphocytes were significantly higher than those in stable patients, both of which were significantly higher than those in control group (all P <0.01). The expression of bc1 2 in LMP1 + cells was higher in LLE patients than in LMP1 cells (P <0.01). There was a positive correlation between the expression of bc1 2 and the expression of LMP1 (r = 0 32 8, P 0 05) in peripheral blood of SLE patients, which was positively correlated with the disease score (r = 0 339 , P <0 0 1). Conclusion EBV-infected B lymphocytes may be involved in the development and progression of SLE by up-regulating the expression of bcl-2 in infected cells and inhibiting the apoptosis of B lymphocytes