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目的研究大肠癌ＡＰＣ／ＭＣＣ基因杂合缺失的作用。方法采用ＰＣＲ技术，并配合限制性片段长度多态现象（ＲＦＬＰ）分析，对４１例外科手术切除大肠癌组织ＡＰＣ／ＭＣＣ基因杂合缺失（ＬＯＨ）进行检测。结果在大肠癌４１例中ＡＰＣ基因属信息个体者２５例，检出ＬＯＨ７例，占２８．０％；ＭＣＣ基因属信息个体者２２例，检出ＬＯＨ８例，占３６．４％。若将ＡＰＣ和ＭＣＣ基因进行综合分析，则信息个体者３６例，检出ＬＯＨ１４例，占３８．９％。ＡＰＣ和ＭＣＣ基因的ＬＯＨ与肿瘤大小、组织学类型、浆膜浸润、淋巴结转移及Ｄｕｋｅｓ分期无关（Ｐ＞０．０５）。结论ＡＰＣ和ＭＣＣ基因ＬＯＨ是大肠癌的常见改变
Objective To study the role of heterozygous deletion of APC/MCC gene in colorectal cancer. Methods PCR was used to detect 41 cases of colorectal cancer APC/MCC gene heterozygosity loss (LOH) in combination with restriction fragment length polymorphism (RFLP) analysis. Results In 41 cases of colorectal cancer, 25 cases were APC gene information individuals, 7 cases were detected as LOH, accounting for 28.0%; 22 cases were MCC gene information individuals, 8 cases were detected as LOH, accounting for 36.4%. If the APC and MCC genes were comprehensively analyzed, 36 informative individuals were detected, and 14 cases of LOH were detected, accounting for 38.9%. The LOH of APC and MCC genes was not associated with tumor size, histological type, serosal infiltration, lymph node metastasis, and Dukes stage (P>0.05). Conclusions LOH of APC and MCC gene is a common change in colorectal cancer