目的 :通过对类风湿性关节炎 (RA)小鼠模型发病不同时期的四肢足关节聚集的T细胞克隆型的分析 ,了解T细胞克隆型与RA病变的关系。方法 :用RT PCR/SSCP法 ,对一种自发性RA小鼠模型 (SKG小鼠 )发病初期与后期四肢足关节聚集的T细胞克隆型进行比较分析。结果 :在发病后期 ,四肢足关节中一致的Vβ2和Vβ8.2T细胞克隆型均明显升高 (分别为 72 .3%和 6 0 .2 % )。结论 :TCR为Vβ2和Vβ8.2的T细胞克隆 ,可能对于SKG小鼠RA的发展起重要作用 OBJECTIVE: To investigate the relationship between T cell clonality and RA pathology through the analysis of clonal T cell clones that accumulate in the limbs and joints of rheumatoid arthritis (RA) mouse models at different stages. Methods: T cell clonotypes of four limbs gathered in the early and late stages of a spontaneous RA mouse model (SKG mouse) were analyzed by RT-PCR / SSCP. Results: At the later stages of pathogenesis, the consistent clones of Vβ2 and Vβ8.2T cells in the foot joints of limbs were significantly elevated (72.3% and 62.2%, respectively). Conclusion: T cell clones with TCR of Vβ2 and Vβ8.2 may play an important role in the development of RA in SKG mice