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斑节对虾(penaeus monodon)幼虾(平均体重4.2～5.5g)的pH低临界值使用盐酸(HCl)醋化海水的静态生物测定的方法来求得.在盐度32ppt时其致死pH(即96hLC_(50)的pH)为3.7(95%置信度是3.4和4.1).最小可采用的pH(此pH在23d中使生长减少5%),在盐度30ppt时估算为5.9.同pH 7.8相比较,长期(23d)置于低pH(4.9)、盐度为30ppt时,对虾干重成分明显减少(P<0.001),而蜕皮频率增加(P<0.05).对虾置于不同pH(5.5或7.8)和盐度(15和30ppt)状况下,低pH(P<0.001)则减少生长,而盐度则不影响生长(P>0.05),但是,pH×盐度相互作用则明显影响其生长(P<0.05).对虾置于不同pH(5.6或7.8)和盐度(15或30ppt)条件下的单因素试验中,血淋巴渗透调节能力在高盐度(JF)<0.001)和低pH(P<0.01)比在低盐度和高pH要更差些,而相互作用并不显著(P>0.05).其致死pH的估算和最小可采用的低pH值,将有助于酸性池对虾养殖的管理.
The low pH threshold for penaeus monodon juveniles (mean body weight 4.2-5.5 g) was determined by static bioassay using hydrochloric acid (HCl) The pH of 96 hLC_ (50)) was 3.7 (95% confidence level was 3.4 and 4.1). The minimum acceptable pH (this reduced the growth of 5% in 23 days) was estimated at 5.9 at 30 ppt salinity. Compared with the control group, the dry weight of shrimps was significantly decreased (P <0.001) and the frequency of molting was increased (P <0.05) at low pH (4.9) and salinity of 30 ppt Or salt stress (15 and 30 ppt), low pH (P <0.001) decreased growth but did not affect salinity growth (P> 0.05). However, pH × salinity interaction significantly affected its growth (P <0.05) .In a single factor experiment with shrimp at different pH (5.6 or 7.8) and salinity (15 or 30 ppt), the hemolymph osmolality was significantly lower at high salinity (JF <0.001) and lower The pH (P <0.01) was worse than that at low salinity and high pH, but the interaction was insignificant (P> 0.05) .The estimation of lethal pH and the minimum acceptable low pH would contribute to the acidity Management of pond shrimp farming.