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目的:检测肾母细胞瘤(nephrohlastoma,wilms tumor,WT)组织中PTEN和MMP_7中的表达。方法:用免疫组化方法检测12例瘤旁正常肾组织和42例WT组织中PTEN、MMP_7的表达。结果:PTEN在WT组织中的表达明显低于正常瘤旁肾组织(P<0.05)。PTEN的表达与性别、年龄和病理学分型无关(P>0.05),而与临床分期明显相关(P<0.05)。12例对照组织标本中MMP_7未见表达,42例WT组织标本中MMP_7在胞浆表达率54.21%。MMP_7在预后良好(favorable histological types,FH)组中的阳性表达率明显低于在预后不良(unfavorable histological types,UH)组中的阳性表达率(P<0.05);PTEN和MMP_7呈负相关。结论:联合检测WT组织中PTEN和MMP_7的表达,能更好评估WT的侵袭和转移等生物学行为,两者可作为判断WT侵袭性的标志物。
Objective: To detect the expression of PTEN and MMP-7 in nephroblastoma (WT) tissues. Methods: The expressions of PTEN and MMP-7 in 12 cases of normal renal tissue and 42 cases of normal tissue were detected by immunohistochemistry. Results: The expression of PTEN in WT tissues was significantly lower than that in normal renal tissues (P <0.05). The expression of PTEN was not related to gender, age and pathological type (P> 0.05), but significantly correlated with clinical stage (P <0.05). MMP-7 was not found in 12 cases of control samples, while in 42 cases of WT samples MMP-7 was 54.21% in cytoplasm. The positive expression rate of MMP_7 in the group of favorable histological types (FH) was significantly lower than that in the group of unfavorable histological types (P <0.05), and the negative correlation between PTEN and MMP_7. Conclusion: Combined detection of PTEN and MMP_7 expression in WT tissue can better evaluate biological behaviors such as invasion and metastasis of WT, both of which can be used as markers to judge the invasiveness of WT.