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目的探讨银翘藿朴汤对CA16病毒感染的支气管上皮细胞中诱导细胞自噬相关因子表达的影响。方法银翘藿朴汤中药液195mg/ml对治疗组16HBE细胞预处理4h,随后将CA16病毒350μl接种至治疗组和对照组16HBE细胞培养基中,Western blot检测TLR7、MyD88、IRF7、IFN等蛋白的表达情况。结果治疗组TLR7、MyD88、IRF7及IFN蛋白水平分别为1.87±0.79、2.32±1.49、4.26±2.37、4.67±2.45,均高于对照组的0.57±0.21、0.69±0.31、1.76±0.69、1.59±0.50(P<0.05)。治疗组病毒VP1蛋白水平为0.31±0.05,低于对照组的0.62±0.05(P<0.05)。结论银翘藿朴汤可以激发机体免疫反应,抑制病毒复制,起到抗病毒作用。
Objective To investigate the effect of Yinqiaohuopu Decoction on the expression of autophagy-related factors induced by CA16 virus in bronchial epithelial cells. Methods 16HBE cells were treated with Yinqiao Huopu decoction liquid 195mg / ml for 4h. Then, 350μl CA16 virus was inoculated into 16HBE cell culture medium and Western blot to detect TLR7, MyD88, IRF7 and IFN Protein expression. Results The protein levels of TLR7, MyD88, IRF7 and IFN in the treatment group were 1.87 ± 0.79, 2.32 ± 1.49, 4.26 ± 2.37 and 4.67 ± 2.45, respectively, which were all higher than those in the control group (0.57 ± 0.21,0.69 ± 0.31,1.76 ± 0.69,1.59 ± 0.50 (P <0.05). The VP1 protein level of the treatment group was 0.31 ± 0.05, which was lower than that of the control group (0.62 ± 0.05, P <0.05). Conclusion Yin Qiao Huopu Decoction can stimulate the body’s immune response, inhibit viral replication, play antiviral effects.