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目的观察CD4~+CD25~(hgih)T淋巴细胞与强直性脊柱炎(AS)发病的相关性,初步探讨调节性T淋巴细胞参与AS发病的可能机制。方法采用双色直接荧光素标记法和多参数流式细胞术检测50例AS患者及20名正常对照外周血CD4~+CD25~+T淋巴细胞和CD4~+CD25~(high)T淋巴细胞的比例,同时收集临床资料及检测外周血IgA、IgG、IgM、红细胞沉降率(ESR)、C反应蛋白(CRP)水平并进行相关分析。结果AS患者外周血CD4~+CD25~+T淋巴细胞与正常对照组差异无统计学意义(P>0.05),CD4~+CD25~(high) T淋巴细胞低于正常对照组(P<0.05),CD4~+CD25~+T淋巴细胞与IgA、IgG、IgM、ESR、CRP、病程、骶髂关节X线分级无相关性(P>0.05)。CD4~+CD25~(high)T淋巴细胞与IgG、IgM、ESR、CRP、病程、骶髂关节X线分级无相关性(P>0.05)。CD4~+CD25~(high)T淋巴细胞与IgA相关(P<0.05)。结论AS患者外周血CD4~+CD25~(high)T淋巴细胞比例降低并与IgA相关,可能参与疾病的发生和发展。
Objective To investigate the correlation between the CD4 ~ + CD25 ~ (hgih) T lymphocytes and the pathogenesis of ankylosing spondylitis (AS) and to explore the possible mechanism of the involvement of regulatory T lymphocytes in the pathogenesis of AS. Methods The proportion of CD4 ~ + CD25 ~ + T cells and CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of 50 patients with AS and 20 normal controls were detected by two-color direct fluorescein labeling and multi-parameter flow cytometry Meanwhile, clinical data and serum IgA, IgG, IgM, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels were collected and analyzed. Results There was no significant difference in CD4 ~ + CD25 ~ + T lymphocytes in peripheral blood between patients with AS and normal controls (P> 0.05). CD4 ~ + CD25 ~ (high) T lymphocytes in AS patients were lower than those in normal control group , CD4 ~ + CD25 ~ + T lymphocytes and IgA, IgG, IgM, ESR, CRP, duration of disease, sacroiliac joint X-ray classification no correlation (P> 0.05). There was no correlation between CD4 ~ + CD25 ~ (high) T lymphocytes and IgG, IgM, ESR, CRP, course of disease and X-ray grading of sacroiliac joint (P> 0.05). CD4 ~ + CD25 ~ (high) T lymphocytes were associated with IgA (P <0.05). Conclusions The proportion of CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of AS patients is decreased and related to IgA, which may be involved in the occurrence and development of the disease.