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目的前期研究发现双特异性磷酸酶5(DUSP5)基因在良性前列腺组织和前列腺癌组织中存在差异性表达,但DUSP5基因的表达与前列腺癌疾病的临床病理相关性尚无统计研究。本次研究检测DUSP5在良性前列腺增生和前列腺癌中的表达,分析其表达水平与临床病理特征的关系。方法通过RT-qPCR检测DUSP5在前列腺癌、前列腺增生组织中DUSP5基因的表达情况;免疫组化检测前列腺癌、前列腺增生标本中DUSP5蛋白的表达。分析DUSP5的表达水平与临床病理特征的关系。结果前列腺癌患者组织中DUSP5基因表达显著下调,DUSP5蛋白表达下调与肿瘤临床分期(≤T2期vs.>T2期:6.43±0.96 vs.5.10±0.70,P<0.01)、Gleason评分(GS<7 vs.GS≥7:6.37±0.78 vs.5.04±0.57,P<0.01)显著相关。结论 DUSP5蛋白在前列腺癌组织中表达下调,检测DUSP5表达水平可协助判断前列腺癌分化程度并评估预后。
OBJECTIVE: To investigate the relationship between DUSP5 gene expression and clinicopathological features of prostate cancer. There is no statistical study on the relationship between the expression of DUSP5 gene and clinicopathological features of prostate cancer. This study examined the expression of DUSP5 in benign prostatic hyperplasia and prostate cancer and analyzed its relationship with clinicopathological features. Methods DUSP5 gene expression in prostate cancer and benign prostatic hyperplasia was detected by RT-qPCR. The expression of DUSP5 protein in prostate cancer and prostatic hyperplasia was detected by immunohistochemistry. The relationship between DUSP5 expression and clinicopathological features was analyzed. Results The expression of DUSP5 was significantly down-regulated in the tissue of patients with prostate cancer. The down-regulation of DUSP5 protein expression was associated with the clinical stage (≤T2 vs. T2: 6.43 ± 0.96 vs.5.10 ± 0.70, P <0.01), Gleason score vs.GS≥7: 6.37 ± 0.78 vs.5.04 ± 0.57, P <0.01). Conclusion DUSP5 protein is down-regulated in prostate cancer tissue. Detecting the expression of DUSP5 may be helpful in judging the differentiation degree of prostate cancer and assessing the prognosis.