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目的研究c-myc和p21在乳腺癌分子亚型中的表达特点及相互关系。方法将1452例乳腺癌标本制成组织芯片,应用免疫组化MaxVision快捷法检测其中c-myc和p21的表达水平。结果根据Nielsen分型,基底样型、Her-2过表达型、裸表达型、luminalA和B型中c-myc的表达率分别为72.8%(147/202)、71.6%(126/176)、63.4%(246/388)、59.5%(235/395)和55.1%(27/49);p21的表达率分别为6%(13/218)、10.9%(20/184)、7.6%(31/407)、16.7%(65/389)和27.5%(14/51)。c-myc在基底样型和Her-2过表达型中表达均高于luminalA(P<0.01)和luminalB型(P<0.05)。p21在ER阴性的基底样型、Her-2过表达型和裸表达型中的表达明显低于ER阳性的lunimalA和B型(P<0.01)。p21的阳性表达与ER相关(P<0.01)。c-myc表达和p21呈负相关(P<0.01)。结论 p21在ER阴性乳腺癌亚型中的表达下调,可能由于c-myc过表达所致,两者相互作用可能参与了ER阴性乳腺癌分子亚型的发生。
Objective To investigate the expression characteristics and relationship of c-myc and p21 in molecular subtypes of breast cancer. Methods 1452 breast cancer specimens were made into tissue microarrays. The expression of c-myc and p21 was detected by MaxVision quick immunohistochemistry. Results According to the Nielsen classification, the expression rates of c-myc in basal, Her-2 overexpression, naked expression, and luminal A and B were 72.8% (147/202) and 71.6% (126/176) respectively. 63.4% (246/388), 59.5% (235/395) and 55.1% (27/49); the expression rate of p21 was 6% (13/218), 10.9% (20/184), and 7.6% (31 /407), 16.7% (65/389) and 27.5% (14/51). The expression of c-myc was higher in basal and Her-2 overexpression than in luminalA (P<0.01) and luminalB (P<0.05). The expression of p21 in ER-negative basal-like, Her-2 over-expression and naked-expression was significantly lower than that of ER-positive lunamal A and B (P<0.01). The positive expression of p21 correlated with ER (P<0.01). There was a negative correlation between c-myc expression and p21 (P<0.01). Conclusion The down-regulation of p21 expression in ER-negative breast cancer subtypes may be due to c-myc over-expression. The interaction between the two may be involved in the occurrence of molecular subtypes of ER-negative breast cancer.