应用 GP-PCR(General primers-PCR)技术,对保存4～6年石蜡包埋宫颈原位癌组织进行HPV 检测,通过对高发区五峰县40例宫颈原位癌及33例宫颈炎的检测,阳性率分别为87.5%和30%,结果表明 GP-PCR 对于检测多型 HPV 感染显示出简便、快速、敏感的优势,适用于大规模 HPV 筛检,对宫颈癌的普查具有重要意义。同时为利用收藏标本回顾性追查 HPV 与宫颈癌之间的关系,提供了实验依据。 HPV-DNA was detected by paraffin-embedded cervical carcinoma in situ 4 to 6 years by GP-PCR (General primers-PCR). Through the detection of 40 cases of cervical carcinoma in situ and 33 cases of cervicitis in Wufeng County, The positive rates were 87.5% and 30%, respectively. The results showed that GP-PCR showed a simple, rapid and sensitive advantage for detecting multi-type HPV infection. It is suitable for large-scale HPV screening and has important significance for the screening of cervical cancer. At the same time, the experimental evidence was provided to retrospectively collect the relationship between HPV and cervical cancer using the collected specimens.