论文部分内容阅读
目的 :为研究CD40分子在人树突状细胞 (DC)抗多发性骨髓瘤 (MM)中的作用及其机制。方法 :采用CD40配体(CD40L)转基因细胞及抗CD40的激发型单克隆抗体在体外激发DC ,并通过DC细胞计数、形态学和细胞表型分析、IL 12定量检测、DC对MM抗原的摄取及混合淋巴细胞反应等手段对其进行研究。结果 :CD40分子配基化可促进DC的体外增殖和分化 ,使DC增加分泌IL 12 ,并下调DC摄取抗原的能力 ,促进DC的成熟 ,同时赋予DC激发自体CD8+细胞增殖的作用 ,使后者对MM细胞产生特异性的杀伤作用。结论 :CD40分子激发不仅有利于DC的增殖、分化和增强激发T细胞增殖 ,而且Th细胞表达的CD40L是DC获得直接激发抗MM抗原特异性CD8+T细胞的关键分子
Purpose : To investigate the role of CD40 in human dendritic cells (DC) against multiple myeloma (MM) and its mechanism. METHODS: CD40 ligand (CD40L) transgenic cells and anti-CD40 activated monoclonal antibodies were used to stimulate DCs in vitro. DC cell counts, morphology and cell phenotype analysis, IL 12 quantitative detection, and DC uptake of MM antigens were used. And mixed lymphocyte reaction and other means to study it. RESULTS: The ligandization of CD40 could promote the proliferation and differentiation of DCs in vitro, increase the secretion of IL 12 by DCs, and down-regulate the ability of DCs to take up antigens, promote the maturation of DCs, and give DCs the ability to stimulate the proliferation of autologous CD8+ cells. Specific killing effect on MM cells. Conclusion :CD40 stimulation is not only beneficial to the proliferation and differentiation of DCs but also enhances the proliferation of activated T cells. Moreover, CD40L expressed in Th cells is a key molecule for DCs to directly stimulate anti-MM antigen-specific CD8+ T cells.