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目的:通过病毒宏基因组学方法分析我国北京市销售的海产品贝类所携带的病毒谱情况。方法:收集北京市销售的330份贝类样本，经过蛋白酶K结合PEG沉淀处理贝类消化腺和肠道后，利用高通量测序和病毒宏基因组进行分析。结果:本研究通过高通量测序后共获得了4 594 270条被注释病毒的基因序列，其中以动物为宿主的病毒序列占21%（965 185/4 594 270）。而在以动物为宿主的病毒序列中，被注释到哺乳动物为宿主的病毒序列占1%（12 205/965 185），包括20个病毒科，包括常见的肠道病毒、甲肝病毒和诺如病毒。诺如病毒基因型包括GII.1、GII.2、GII.13、GII.14和GII.17，GII.2和GII.17诺如病毒基因序列与人间同型流行株的核苷酸相似性分别为100%和98%。结论:北京市销售的不同种贝类中含有多种可以引起人类疾病的病毒病原体序列，有必要开展相应的监测和进一步分析病毒生物活性。“,”Objective:The virus spectrum in sea shellfish sold in Beijing, China was analyzed by the virus metagenomic approach.Methods:A total of 330 samples of shellfish were collected in Beijing. The digestive glands and intestines of shellfish were treated by protease K and PEG precipitation. The high-throughput sequencing and virus metagenomics analysis were performed.Results:In this study, 4 594 270 annotated viral gene sequences were obtained by high-throughput sequencing, among which the sequences from viruses of animal host accounted for 21% (965 185/4 594 270). Among these sequences from viruses of animal host, 1% (12 205 / 965 185) were annotated to viruses of mammalian hosts, belonging to 20 virus families, including common enteroviruses, hepatitis A virus, and norovirus. The genotypes of norovirus included GII. 1, GII. 2, GII. 13, GII. 14 and GII. 17. The GII.2 and GII.17 noroviruses shared 100% and 98% nucleotide similarity with the human stains of respective genotype in human.Conclusions:Different species of shellfish sold in Beijing contained multiple sequences of viral pathogens that can cause human diseases, and it is necessary to carry out the corresponding surveillance and the further analysis of viral biological activity.