论文部分内容阅读
目的建立南通地区C型乙型肝炎病毒(HBV)流行株PreS基因参考序列。方法扩增25例HBV慢性无症状携带者的PreS基因,分析并确定基因型,选同HBVC亚型的PreS拟定参照序列。结果将所测基因序列与HBV不同型PreS序列用Clustalx1.8软件对比,经MEGA3.1软件分型。结果表明,其中有19份血样属C型,有6份血样属B型;南通地区C型HBV流行株的PreS参照序列与GenBank收录的HBV-C型序列D50517.1以及重庆地区的参照序列进行比较,分别有6个核苷酸和10个核苷酸位点的差异,同源性分别为98.9%和98.1%。结论建立了南通地区C型HBV流行株的PreS基因参照序列。
Objective To establish a PreS gene reference sequence of hepatitis C virus (HBV) of type C in Nantong area. Methods The PreS gene of 25 chronic HBV carriers was amplified and analyzed. The genotype was determined and the PreS reference sequence of HBV subtype was selected. Results The sequence of the tested gene was compared with the different preS sequences of HBV using the Clustalx1.8 software and the MEGA3.1 software. The results showed that 19 of the blood samples were of type C and 6 of them were of type B. The PreS reference sequence of the C-type HBV epidemic strains in Nantong region was identical with the HBV-C sequence D50517.1 in GenBank and the reference sequences in Chongqing Compared with 6 nucleotide and 10 nucleotide respectively, the homologies were 98.9% and 98.1% respectively. Conclusion The PreS gene reference sequence of C-type HBV in Nantong area was established.