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目的：了解氧化砷（Ａｓ２Ｏ３）治疗急性早幼粒细胞白血病（ＡＰＬ）的细胞和分子机制。方法：以早幼粒细胞白血病细胞株ＮＢ４细胞等为研究对象，利用流式细胞仪，ＤＮＡ电泳，Ｎｏｒｔｈｅｒｎ、Ｗｅｓｔｅｒｎ印迹，免疫组化等手段，观察Ａｓ２Ｏ３对ＡＰＬ的作用。结果：Ａｓ２Ｏ３能显著诱导ＮＢ４细胞凋亡，而不影响ＨＬ－６０和Ｕ９３７的生长和存活。氧化砷能有效降低ＮＢ４细胞中ｂｃｌ－２基因的表达，而对其它几种凋亡相关基因（包括ｐ５３、ｃ－ｍｙｃ、ｂａｘ和ｂｃｌ－ＸＬ）的ｍＲＮＡ水平无影响。结论：这可能是Ａｓ２Ｏ３诱导ＮＢ４细胞凋亡的分子机制之一。
Objective: To understand the cellular and molecular mechanisms of arsenic oxide (As2O3) in the treatment of acute promyelocytic leukemia (APL). METHODS: The effects of As2O3 on APL were observed by means of flow cytometry, DNA electrophoresis, Northern blot, Western blot and immunohistochemistry. Results: As2O3 can induce apoptosis of NB4 cells without affecting the growth and survival of HL-60 and U937. Arsenic oxide can effectively reduce the expression of bcl-2 gene in NB4 cells, but has no effect on the mRNA levels of other apoptosis-related genes (including p53, c-myc, bax, and bcl-XL). Conclusion: This may be one of the molecular mechanisms of As2O3-induced apoptosis in NB4 cells.