通过采用蛋白质组学方法分析酵母家族蛋白激酶的研究中发现:半胱氨酸残基上存在多种蛋白修饰形式,其中3种可能为蛋白质翻译后修饰,9种可能来自于体外反应,主要由体外的化学衍生反应生成。通过二级质谱数据解析和生物信息学方法,研究了激酶家族蛋白质半胱氨酸残基上修饰的种类和化学结构,以及可能的反应过程,并对这些修饰出现的频率进行了归类分析。 The analysis of yeast family protein kinases using proteomics revealed that many protein modifications exist on cysteine ​​residues, three of which may be post-translational modifications of proteins, nine of which may be due to in vitro responses consisting essentially of In vitro chemical derivatization. Through the analysis of MS data and bioinformatics methods, the types and chemical structures of the cysteine ​​residues on the kinases’ family proteins and the possible reaction processes were studied. The frequencies of these modifications were also analyzed.