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为研究血管内皮细胞生长因子(VFGF)在卵巢浆液性囊腺癌癌细胞中的表达及其与组织病理学参数、无瘤生存期和肿瘤细胞增殖的关系,选择1991～1993年意大利Ancona大学妇产科学院收治根据WHO诊断标准确诊的卵巢浆液性囊腺癌患者32例作为研究对象,10例绝经后浆液性囊腺瘤患者和10例卵巢正常的绝经后妇女作对照。根据FIGO分期标准进行分期,手术范围包括全子宫、双附件、大网膜切除和淋巴结取样。术后均行联合化疗(顺铂60mg/m~2,环磷酰胺600mg/m~2和表柔比星60mg/m~2),术后随访至1997年12月。 采用免疫组化SP法进行VEGF和MIB1的染色,福尔马林固定和石蜡包埋的组织切片厚度3μm,
To investigate the expression of vascular endothelial growth factor (VFGF) in ovarian serous cystadenocarcinoma cells and its relationship with histopathological parameters, tumor-free survival and tumor cell proliferation, we selected women from Ancona University in Italy from 1991 to 1993 Obstetrics and Gynecology School admitted 32 cases of ovarian serous cystadenocarcinoma diagnosed according to WHO diagnostic criteria as the research object, 10 cases of postmenopausal serous cystadenoma and 10 cases of ovarian normal postmenopausal women as a control. Staging according to the FIGO staging criteria included total hysterectomy, double attachment, omental resection, and lymph node sampling. Postoperative chemotherapy (cisplatin 60mg / m ~ 2, cyclophosphamide 600mg / m ~ 2 and epirubicin 60mg / m ~ 2) were followed up until December 1997. Immunohistochemical SP method for VEGF and MIB1 staining, formalin-fixed and paraffin-embedded tissue sections thickness 3μm,