A novel pulsed-electrospray device is reported in this paper. With such an apparatus, the observation of the electrospray process under the pulsed high voltage conditions has been successfully made with microscope, oscilloscope and mass spectrometer. By using pulsed spray technique, the sample flow rate can be averagely decreased to ＜100 nL/min. After increasing the frequency of pulsed high voltage, it is hopefully to directly combine this device to time-of-flight mass spectrometer and obtain a great enhancement both in signal-to-noise ratio and in detection sensitivity for mass spectrometry analysis.