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目的利用荧光光谱法研究Eu3+-美他环素(MTC)-溴化十六烷基三甲铵(CTMAB)体系的稀土发光效应和最佳形成条件,并对其作用机制进行探讨。方法建立测定MTC的荧光分光光度法,探讨在最佳实验条件下不同浓度的MTC和MTC配合物分别对大肠杆菌和金黄色葡萄球菌的抑菌效果。结果实验结果表明,在pH 9.0～10.5 Na2B4O7-NaOH的缓冲溶液中,美他环素和阳离子表面活性剂溴化十六烷基三甲铵对Eu3+的荧光具有显著协同增敏作用,当MTC浓度为0.45～12μg.mL-1时,荧光强度与浓度呈良好的线性关系。结论据此建立了测定药物制剂中美他环素含量的荧光光谱法,方法的检出限0.015 3μg.mL-1,标准偏差1.03%,不同浓度的美他环素和美他环素配合物对大肠杆菌抑菌效果较好,当美他环素浓度为1×10-3mol.L-1时对大肠杆菌抑菌效果最好。
OBJECTIVE To study the rare earth luminescence and optimum formation conditions of Eu3 + -metastatocyclyl (MTC) -dodecyltrimethylammonium bromide (CTMAB) system by fluorescence spectroscopy and its mechanism of action. Methods To establish a fluorescence spectrophotometric method for the determination of MTC, and to explore the antibacterial activity of different concentrations of MTC and MTC complexes against Escherichia coli and Staphylococcus aureus under the optimal experimental conditions. Results The experimental results showed that, in pH 9.0 ~ 10.5 Na2B4O7-NaOH buffer solutions, the synergistic sensitization effect of mestatin and cationic surfactant cetyltrimethylammonium bromide on Eu3 + fluorescence was obvious. When MTC concentration was 0.45 ~ 12μg.mL-1, the fluorescence intensity and concentration showed a good linear relationship. Conclusion The fluorescence spectrometry method was established for the determination of the content of mesotaxel in pharmaceutical preparations. The detection limit of this method was 0.015 3 μg.mL-1 with a standard deviation of 1.03%. Different concentrations of mesotaxel and mesotrione The bacteriostatic effect of E.coli is better, when the concentration of me tricalcium is 1 × 10-3mol.L-1, the antibacterial effect of E.coli is the best.