论文部分内容阅读
目的 :从基因转录水平探讨ＡｎｇⅡ、ｍｍ -ＬＤＬ及ＴＮＦα对ＥＣＶ30 4细胞ＰＤＧＦ -Ｂ链基因表达的影响。方法 :( 1 )以ＧＡＰＤＨ为内参照 ,用反转录 -多聚酶链反应 (ＲＴ -ＰＣＲ)方法检测 1 0 - 6 ｍｏｌ/ＬＡｎｇⅡ、1 0 0 μｇ/ｍＬｍｍ -ＬＤＬ及 2 0 0Ｕ/ｍＬＴＮ?
OBJECTIVE: To investigate the effect of AngⅡ, mm-LDL and TNFα on the gene expression of PDGF-B chain in ECV304 cells by gene transcription. Methods: (1) GAPDH was used as an internal reference, and 1 0-6 mol / LAngⅡ, 100 μg / mLmm-LDL and 200U / mL TNT were detected by reverse transcription polymerase chain reaction (RT-PCR)