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目的:观察巨噬细胞在小鼠肾纤维化进展期和恢复期的作用。方法:采用单侧输尿管结扎(UUO)肾纤维化模型和输尿管再通模型(RUUO)进行试验研究;用Masson染色和HE染色观察肾脏纤维化程度和炎症变化趋势;流式分析肾脏中巨噬细胞细胞群的比例变化。结果:Masson染色显示肾脏纤维化程度在梗阻解除后胶原沉积面积减轻从80%降到46%,差异有统计学意义(P<0.05)。HE染色显示梗阻解除后肾间质炎症减轻,且有新生小管形成。流式结果显示梗阻解除后巨噬细胞细胞群比例由19%降到2.6%,差异有统计学意义(P<0.05)。结论:巨噬细胞可能在肾纤维化恢复期发挥一定作用。
Objective: To observe the role of macrophages in the progression and recovery of renal fibrosis in mice. Methods: Unilateral ureteral obstruction (UUO) model of renal fibrosis and ureteral recanalization model (RUUO) were used to study the experimental study. Masson staining and HE staining were used to observe the changes of renal fibrosis and inflammation. Flow cytometry analysis of renal macrophages The proportion of cell population changes. Results: Masson staining showed that the extent of renal fibrosis was reduced from 80% to 46% after obstruction was relieved, the difference was statistically significant (P <0.05). Hematoxylin and eosin staining showed that renal interstitial inflammation was relieved after obstruction was relieved, and neo-tubule formation occurred. The results of flow cytometry showed that the percentage of macrophage cell population decreased from 19% to 2.6% after obstruction was removed, the difference was statistically significant (P <0.05). Conclusion: Macrophages may play a role in the recovery of renal fibrosis.