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目的：在大肠杆菌高效表达抗胃癌单抗３Ｈ１１的ｓｃＦｖ。方法：利用ＰＣＲ技术将３Ｈ１１ｓｃＦｖ基因从分泌型表达载体转移至高效表达载体，获得３Ｈ１１ｓｃＦｖ包含体的表达，经超声裂解、洗涤、变性后，尝试透析复性和凝胶过滤色谱柱上在位复性，结果：透析复性未能得到有活性的３Ｈ１１ｓｃＦｖ，而柱上在位复性效果良好，获得有功能性的３Ｈ１１ｓｃＦｖ。结论：成功进行了３Ｈ１１ｓｃＦｖ的柱上复性，不同ｓｃＦｖ在表达和复性中显示出明显差异，在基因工程抗体研制中值得注意。
Objective: To efficiently express scFv against gastric cancer monoclonal antibody 3H11 in E. coli. METHODS: The 3H11 scFv gene was transferred from the secretory expression vector to the high-level expression vector by PCR to obtain the expression of the 3H11 scFv inclusion body. After ultrasonication, washing, and denaturation, try renaturation and refolding on the gel filtration column. RESULTS: The dialysis renaturation failed to obtain active 3H11 scFv, while the on-column refolding effect was good and a functional 3H11 scFv was obtained. Conclusion: The renaturation of 3H11scFv was successfully performed. Different scFv showed significant differences in expression and renaturation. It is worthy of attention in the development of genetically engineered antibodies.