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对29种药材进行细胞毒筛选试验时,发现吴茱萸Evodia rutaecarpa 果实的乙醇提取物对中国仓鼠肺细胸(V-79)有显著作用。报道了从该植物中分离并鉴定的细胞毒素。该乙醇提取物的水溶液相继用氯仿及正丁醇处理后,对V-79有细胞毒活性的物质主要集中在氯仿次级萃取液中,将其再分馏成几个部分,以生物测定监控以取得细胞毒部分(Fr-B8),其lC_(50)值<3.0μg/ml。Fr-B8再上硅胶G柱HPLC得到化合物(1)和(2)。(1)为淡黄色针晶,mp267～269℃,(2)为黄色粉末,mp262～264℃。两者的碘化铵钾试验均
When cytotoxic screening tests were conducted on 29 medicinal materials, it was found that the ethanol extract of Evodia rutaecarpa fruit had a significant effect on Chinese hamster lung thin chest (V-79). The cytotoxins isolated and identified from this plant are reported. After the aqueous solution of the ethanol extract is successively treated with chloroform and n-butanol, substances having cytotoxic activity on V-79 are mainly concentrated in the chloroform secondary extract, and then fractionated into several fractions and monitored by bioassay. The cytotoxic fraction (Fr-B8) was obtained and its lC_(50) value was <3.0 μg/ml. Fr-B8 was further subjected to HPLC on a silica gel column to give Compounds (1) and (2). (1) Light yellow needles, mp 267-269°C, (2) yellow powder, mp 262-264°C. Both ammonium iodide tests