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参加研究的是351名在大学研究门诊作常规精液检查的病人,用手淫法获精液标本,收集在无菌塑料容器内,分析前在37℃条件下液化20～30分钟。用巴氏染色(Papanicolaal-Stained)的精液涂片评价精子形态,并由一有经验的操作人员用亮视野照明(×1000)读数,然后以Jouannet多异常精子指数评价100个精子中异常数并评分。作者把它们分为头、中段和尾部缺陷三类。根据WHO标准,病人分为正常精子<50%(畸形精子组)和>50%(正常精子组)两组。为了找出特殊形态缺陷与白细胞小集团之间的可能关系,作者又按各种特殊缺陷分为>50%和<50%组。对351例病人作了精液分析,并用免疫
Participants in the study were 351 patients in the University Research Outpatient Department for routine semen examination. Semen samples were obtained by masturbation and collected in sterile plastic containers. The samples were liquefied at 37 ° C for 20-30 minutes prior to analysis. Sperm morphology was assessed by Pap smear of Papanicolal-Stained and illuminated (* 1000) with a bright field of vision by an experienced operator, followed by evaluation of the anomalies in 100 spermatozoa with the Jouannet Multi-Abnormal Sperm Index score. The authors classify them into three categories, head, middle and tail defects. According to WHO criteria, patients were divided into normal sperm <50% (abnormal sperm group) and> 50% (normal sperm group) two groups. In order to find out the possible relationship between special morphological defects and leukocyte subgroups, the authors divided into groups of> 50% and <50% according to various special defects. 351 patients were semen analysis, and immunization