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采用Ｍａｎｊｕｎａｔｈ等［１］设计的仅对人型、牛型结核杆菌及卡介苗的ＤＮＡ起反应的一对引物，对４０例结核性脑膜炎患儿脑脊液中结核杆菌的ＤＮＡ进行聚合酶链反应（ＰＣＲ）检测，并与涂片、培养比较，阳性率分别为６０％、０、１０％。对照组８例非结核性脑膜炎患儿的脑脊液经ＰＣＲ检测结果均阴性。为确定该引物的特异性，分别扩增１７种分支杆菌的ＤＮＡ，除人型、牛型结核杆菌及卡介苗的ＤＮＡ出现２４０ｂＰ扩增带外，其它分支杆菌均未出现该扩增带。本文结果表明，应用该引物检测结核性脑膜炎患儿脑脊液中结核杆菌，具有较高的特异性及敏感性，为儿童结核性脑膜炎提供了快速、敏感、特异的病原学诊断方法
Using a pair of primers designed by Manjunath et al [1] to react only with DNA of human, Mycobacterium bovis and BCG, DNA of M. tuberculosis in CSF of 40 children with tuberculous meningitis was subjected to polymerase chain reaction (PCR) ) Test, and compared with the smear, culture, the positive rates were 60%, 0,10%. CSF in 8 children with non-tuberculous meningitis in the control group was negative by PCR. In order to determine the specificity of the primers, 17 kinds of DNA were amplified from mycobacteria, respectively. Except for the 240bP amplification band of DNA of human, Mycobacterium bovis and BCG, none of the other mycobacteria appeared. The results show that the application of this primer in children with tuberculous meningitis in cerebrospinal fluid of Mycobacterium tuberculosis has a high specificity and sensitivity for children with tuberculous meningitis provides a rapid, sensitive and specific etiological diagnosis